METHOD

Multimodal optical coherence tomography in the assessment of cancer treatment efficacy

Sirotkina MA1, Kiseleva EB1, Gubarkova EV1, Buyanova NL1, Elagin VV1, Zaitsev VYu1,2, Matveev LA1,2, Matveev AL1,2, Kirillin MYu2, Gelikonov GV1,2, Gelikonov VM1,2, Kuznetsov SS1, Zagaynova EV1, Gladkova ND1
About authors

1 Department of Microbiology and Immunology,
Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia

2 Institute of Applied Physics, Russian Academy of Sciences, Nizhny Novgorod, Russia

Correspondence should be addressed: Marina Sirotkina
pl. Minina i Pozharskogo, d. 10/1, Nizhny Novgorod, Russia, 603950; ur.liam@m_aniktoris

About paper

Funding: this work was supported by the Ministry of Education and Science of the Russian Federation (grant no. 14.B25.31.0015), numerical processing of CP OCT images was supported by grant no. 15-32-20250 of the Russian Foundation for Basic Research, algorithm modification and development of software/ hardware OCT-system for mapping microcirculation and elastography images was supported by the grant of the President of the Russian Federation for young scientists (no. MK-6504.2016.2) and the grant of the Russian Foundation for Basic Research (no. 16-02-00642-а).

Acknowledgements: authors thank professor Alex Vitkin of University of Toronto (Toronto, Canada), the leading scientist of the Russian Federation Government Mega-grant 14.B25.31.0015.

Received: 2016-08-15 Accepted: 2016-08-25 Published online: 2017-01-05
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One of the challenges faced by modern medicine is finding new methods of functional imaging of biological tissues in patients that allow detection of early tumor response to treatment. One of such methods proposed in this work is multimodal optical coherence tomography (MM OCT). It combines cross-polarization OCT (CP OCT) for visualization of tissue structure and assessment of connective tissue health, OCT-based microangiography (OCT MA) for visualization of the vasculature, and OCT- based elastography for measuring tissue stiffness. The efficacy of this method was tested during the course of photodynamic therapy (PDT), as major PDT targets are cellular and vascular components of a tumor. The experiments were carried out on the CT26 colon carcinoma transplanted into the mouse ear. It was shown that the efficacy of PDT can be assessed using MM OCT. For example, CP OCT can help differentiate between necrotic and intact tumors; OCT MA detects blood circulation defects that lead to slower blood circulation or circulatory stagnation followed by tumor death. OCT-based elastography is helpful in assessing stiffness of normal and pathological tissues.

Keywords: photodynamic therapy, multimodal optical coherence tomography, cross-scattering, microangiography, elastography, CT26 colon carcinoma, experimental tumor

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