DOI: 10.24075/brsmu.2017-01-09

ORIGINAL RESEARCH

Wound care with the leaf extract of cecropin P1-producing transgenic kalanchoe: histological findings

Belous AS1,2, Shevelev AB1, Trubnikova EV1, Biryukova YuK1, Mishina ES2, Loyko EA2, Lebedeva AA3, Zakharchenko NS3
About authors

1 Research Laboratory “Genetics”,
Kursk State University, Kursk, Russia

2 Kursk State Medical University, Kursk, Russia

3 Puschino branch of M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry of RAS, Puschino, Russia

Correspondence should be addressed: Alexandr Belous
prospect A. Deriglazova, d. 71, kv. 89, Kursk, Russia, 305014; ur.liam@suoleb.s.a

About paper

Funding: this work was supported by the Ministry of Education and Science of the Russian Federation (Grant No. 14.607.21.0016 dated June 5, 2014, ID RFMEFI60714X0016).

Acknowledgements: authors thank Viktor Lazarenko of Kursk State Medical University and Alexandr Khudin of Kursk State University for providing full access to the research facilities of both universities.

Contribution of the authors to this work: Belous AS, Zakharchenko NS — research planning, data collection; Shevelev AB — analysis of literature, data interpretation; Trubnikova EV — data interpretation, statistical analysis; Biryukova YuK — data interpretation, drafting a manuscript; Mishina ES, Loyko EA — data collection; Lebedeva AA — analysis of literature. All authors participated in editing of the manuscript.

Received: 2017-02-05 Accepted: 2017-02-18
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Management of purulent wounds is a problem that requires particular attention: wounds are a common injury type for which suppurative complications are frequent, mortality rates are high and antimicrobial therapy may be ineffective due to the presence of drug-resistant bacteria in the wound. In this work we have studied the effectiveness of wound treatment with the leaf extract of transgenic Kalanchoe pinnata modified to produce antimicrobial peptide cecropin P1. Purulent wounds infected with Staphylococcus aureus were modeled in Wistar rats. Four groups of animals were formed, with 10 animals in each group. In all groups, the wounds were cleansed with 3 % hydrogen peroxide solution once a day; all groups except the controls received additional treatment. Group 2 received 10 % cefazolin solution, group 3 received kalanchoe juice, group 4 received the juice of cecropin P1-producing kalanchoe. Histologic stains of biopsy samples were performed after rats were sacrificed by anesthetic overdose on days 3, 10 and 14 after treatment onset. On day 3, wound dynamics was the same in all groups. On day 10 exudate was still observed in the controls; in group two exudation was almost finished and regeneration was about to begin; in groups 3 and 4 the wound defect was filled with granulation tissue. In spite of epidermal repair along the wound edges in groups 2 and 3, there still was some sloughing and granulation tissue was less mature than in group 4. We recommend conducting more extensive clinical research of the leaf extract of cecropin P1-containing transgenic Kalanchoe pinnata.

Keywords: wound healing, purulent wound, Staphylococcus aureus, Kalanchoe pinnata, cecropin P1, antimicrobial treatment

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