BIOMEDICAL JOURNAL OF PIROGOV RNRMU (MOSCOW, RUSSIA)
The use of antimicrobial photodynamic therapy mediated by MC540 in the infected wound model
Photodynamic therapy (PDT) is an alternative to conventional therapies of infections. It can kill drug-resistant bacteria without damaging host tissues. In the present work we use the multiresistant strain PA21 of Pseudomonas aeruginosa to model a wound infection in mice and study the effect of PTD mediated by aqueous solutions of the anionic photosensitizer merocyanine 540 (solubilized in water and in 0.25 M sodium chloride) on bacterial decontamination and wound healing. To assess a therapeutic effect of PDT, we monitored bacterial contamination of the wound, measured the wound size in two planes using a caliper and carried out a histopathological examination of infected tissue sections. Our study reveals that PDT mediated by MC540 in the sodium chloride solution can induce bacterial death, inhibit bacterial re-growth and accelerate wound healing.
Keywords: antimicrobial photodynamic therapy, multiresistant clinical strain, Pseudomonas aeruginosa, skin infection, soft tissue infection, wound healing, merocyanine 540, wound infection