Fag. 1.Physical and chemical properties of endolysin L-KPP10 and artilysin AL-KPP10. Circular dichroism spectra of AL-KPP10 and L-KPP10 (A). Spectra of tryptophan fluorescence of proteins under denaturing and native conditions (B). Dynamic laser light scattering in the solutions of native AL-KPP10 (C) and L-KPP10 (D)

Fig. 2. Antimicrobial activity of the recombinant endolysin against strain РА103 of Pseudomonas aeruginosa. Treatment with different concentrations of the recombinant L-KPP10 in the absence of permeabilizing agents (A). 0.5 mM EDTA added to the bacteria combined with L-KPP10 (B). * — Difference is significant at р = 0.05 (Mann-Whitney U)

Fig. 3. Antimicrobial activity of the recombinant artilysin AL-KPP10 against sensitive strains of Pseudomonas aeruginosa. Treatment of Pseudomonas aeruginosa sensitive strains with different concentrations of recombinant AL-KPP10 in the absence of permeabilizing agents (А), р < 0.01 (Mann-Whitney U). Electron microscopy of Ts 43-16 (clinical isolate of Pseudomonas aeruginosa) following incubation with 50 μg/ml AL-KPP10 for 10 min, 20 min and 30 min (B). A Petri dish with artilysin and clinical isolate Ts 49-16 of Pseudomonas aeruginosa upon incubation for 30 min (C)