DOI: 10.24075/brsmu.2017-06-12


Temporal dynamics of cytokines in the blood of rats with experimentally induced autoimmune encephalomyelitis

Pozdniakova NV1, Turobov VI2, Garanina EE3, Ryabaya OA1, Biryukova YuK4, Minkevich NI2, Trubnikova EV5, Shevelev AB6, Kuznetsova TV7, Belyakova AV4, Udovichenko IP2,8
About authors

1 N. N. Blokhin National Medical Research Center of Oncology, Moscow, Russia

2 Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Pushchino branch, Russia

3 Кazan (Volga Region) Federal University, Kazan, Russia

4 Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, Moscow, Russia

5 Kursk State University, Kursk, Russia

6 Emanuel Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow, Russia

7 Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia

8 Pushchino State Institute of Natural Sciences, Pushchino, Russia

Correspondence should be addressed: Igor Udovichenko
Pr-t Nauki, d. 6, Puschino, Moscow oblast, Russia, 142290; ur.xednay@1oknehcivodui

About paper

Funding: this work was supported by the Ministry of Education and Science of the Russian Federation (Grant agreement 14.607.21.0133 dated October 27, 2015, ID RFMEFI60715X0133).

Acknowledgements: the authors thank Boris Shevelev for help in immunization of the animals.

All authors' contribution to this work is equal: selection and analysis of literature, research planning, data collection, analysis, and interpretation, drafting of a manuscript, editing.

Received: 2017-12-15 Accepted: 2017-12-20

In this work we explore the temporal dynamics of cytokines in Dark Agouti rats with experimentally induced autoimmune encephalomyelitis (EAE). The main group consisted of 11 animals who were injected with 100 μl (per leg) of spinal cord homogenate obtained from random-bred rats and combined with incomplete Freund’s adjuvant to the hind footpads. The control group included 7 animals who received 100 μl of normal saline mixed with incomplete Freund’s adjuvant. Blood samples (500 μl) were collected daily, starting from day 1 through day 7. We ran a Bio-Plex-based multiplex cytokine assay on the samples using the Bio-Plex Pro Rat Cytokine 24-plex Assay kit. EAE in rats was shown to simulate progression of multiple sclerosis in humans in terms of temporal dynamics of lymphoproliferative and hematopoietic factors IL-1b, IL-2, IL-4, IL-5, IL-6, and IL-7. The studied model satisfactory imitates the dynamics of factors stimulating migration of lymphocytes, monocytes and other immune cells, including IL-17, RANTES (CCL-5) and MCP-1 (CCL-2) but excluding GRO/KC (CXCL1), which shows a different dynamics. The model also resembles patterns of human multiple sclerosis in terms of factors affecting cytotoxic and apoptotic reactions, including IFNγ, IL-6 and IL-17, but excluding TNFα.

Keywords: multiple sclerosis, immunization, experimental autoimmune encephalomyelitis, myelin, multiplex cytokine assay