ISSN Print 2500–1094
ISSN Online 2542–1204
Bulletin of RSMU
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Temporal dynamics of cytokines in the blood of rats with experimentally induced autoimmune encephalomyelitis
About authors
1 Blokhin National Medical Research Center of Oncology, Moscow
2 Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Pushchino branch, Russia
3 Кazan (Volga Region) Federal University, Kazan, Russia
4 Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, Moscow
5 Kursk State University, Kursk
6 Emanuel Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow, Russia
7 Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia
8 Pushchino State Institute of Natural Sciences, Pushchino, Russia
Correspondence should be addressed: Igor Udovichenko
Pr-t Nauki, d. 6, Puschino, Moscow oblast, Russia, 142290; ur.xednay@1oknehcivodui
About paper
Funding: this work was supported by the Ministry of Education and Science of the Russian Federation (Grant agreement 14.607.21.0133 dated October 27, 2015, ID RFMEFI60715X0133).
Acknowledgements: the authors thank Boris Shevelev for help in immunization of the animals.
All authors' contribution to this work is equal: selection and analysis of literature, research planning, data collection, analysis, and interpretation, drafting of a manuscript, editing.
Received: 2017-12-15
Accepted: 2017-12-20
Published online: 2018-01-25
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Fig. 1. Changes in cytokine levels in the blood serum of rats with induced EAE in comparison with the controls 1 day after the injection. Cytokine levels in the controls were taken as 100 %. Significant differences are marked with arrows
Fig. 2. Changes in cytokine levels in the blood serum of rats with induced EAE in comparison with the controls 5 days after the injection. Cytokine levels in the controls were taken as 100 %. Significant differences are marked with arrows
Fig. 3. Changes in cytokine levels in the blood serum of rats with induced EAE in comparison with the controls 7 days after the injection. Cytokine levels in the controls were taken as 100 %. Significant differences are marked with arrows
Table 1. Significance of differences between the main and the control groups of animals calculated by using the Mann–Whitney test with Yates's correction for continuity. Hypothesis tested: the absence of significant differences between the samples. The result is presented as Fisher’s p with three significance thresholds: p > 0.05 — the difference is insignificant; 0.01 < р ≤ 0.05 — the first significance threshold; 0.001 < р ≤ 0.01 — the second significance threshold; р ≤ 0.001 — the third significance threshold
Table 2. Statistical analysis of changing cytokine levels in the main group of rats with induced autoimmune encephalomyelitis and the controls
