DOI: 10.24075/brsmu.2018.010


Physical and chemical properties of recombinant kpp10 phage lysins and their antimicrobial activity against Pseudomonas aeruginosa

Antonova NP1,2, Balabanyan VYu2, Tkachuk AP1, Makarov VV3, Guschin VA1,3,4
About authors

1 Laboratory for Translational Biomedicine,
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology, Moscow

2 Faculty of Fundamental Medicine,
Lomonosov Moscow State University, Moscow

3 Center for Strategic Planning of the Ministry of Health of the Russian Federation, Moscow

4 Department of Virology, Faculty of Biology,
Lomonosov Moscow State University, Moscow

Correspondence should be addressed: Vladimir Gushchin
Gamaleya 18, Moscow, 123098; moc.liamg@adainawow, gro.ayelamag@nihchsug.a.rimidalv

About paper

This work was supported by the Ministry of Education and Science of the Russian Federation as part of the project RFMEFI60117X0018.

Received: 2018-02-02 Accepted: 2018-03-23

One of the most promising approaches to combatting multiple drug resistance is based on the use of recombinant endolysins. In this work we study physical, chemical and lytic properties of phage KPP10, a recombinant endolysin effective against Pseudomonas aeruginosa at concentrations of 3 μg/ml. Fused with a fragment of positively charged SMAP-29 peptide into one reading frame, artilysin KPP10 is effective against laboratory strains and clinical isolates of Pseudomonas aeruginosa in the absence of permeabilizers. Our findings encourage preclinical trials of this artilysin in infectious models

Keywords: nosocomial infections, antimicrobial resistance, bacteriophages, lysins of bacteriophages, phage KPP10, artilysin KPP10