BIOMEDICAL JOURNAL OF PIROGOV RNRMU (MOSCOW, RUSSIA)
Observing the dynamics of the NAD+ to NADH ratio in Danio rerio embryo tissues using a genetically encoded biosensor
The zebrafish Danio rerio is a popular model species for biomedical research focusing on modeling human diseases and screening of therapeutic drugs. Genetically encoded biosensors based on fluorescent proteins are widely used in many laboratories worldwide to study the biology of living systems of various complexity in vivo. The main advantage of these molecular tools is that they allow observing biological processes in intact systems in real time. In the present work we study the dynamics of the intracellular ratio of NAD+ to NADP in the yolk sac and tissues of D. rerio larvae using the SoNar biosensor following the injection of glycolisis end products, lactate and pyruvate.
Keywords: genetically encoded biosensors, Danio rerio, pyruvate, lactate