DOI: 10.24075/brsmu.2018.014


Expression of steroid hormone receptors in the tissue of endometriomas in first-time and relapsing patients

Bulatova LS1, Solomatina AA1, Kareva EN2, Kotsyubinskaya NA2
About authors

1 Department of Obstetrics and Gynecology, Pediatrics Faculty,
Pirogov Russian National Research Medical University, Moscow, Russia

2 Department of Molecular Pharmacology and Radiobiology named after Academician P.V.Sergeev, Medical-Biological Faculty,
Pirogov Russian National Research Medical University, Moscow, Russia

Correspondence should be addressed: Lolita Bulatova
Ostrovityanova 1, Moscow, 117997; ur.liam@78.avotalub.atilol

Received: 2017-10-30 Accepted: 2018-04-13

A molecular marker for the risk of endometriosis recurrence can help to customize a post-operative treatment plan for an individual patient. The aim of this study was to compare the expression of genes coding for estradiol (mER, ERα, ERβ) and progesterone (PGRmC1, mPR, РR-А, PR-B) receptors in first-time and relapsing patients with ovarian endometriosis. Our study included 94 women of reproductive age with ovarian endometriosis: 82 first-time and 12 relapsing patients. The expression of genes coding for steroid receptors was measured using reverse transcription polymerase chain reaction. Recurrent conditions were characterized by a change in the expression of estrogen receptors and unchanged expression of progesterone receptors. Expression of mER in the tissue of patients with first-time endometriosis was 15.09 ± 1.18. Patients undergoing recurrence demonstrated a 3-fold increase in mER expression (from 15.09 ± 1.18 to 44.45 ± 9.1). Also, in such patients ERβ expression was 5 times higher increasing from 11.71 ± 0.22, which is an average value for first-time patients, to 10.02 ± 3.81, while ERα expression surged 7-fold from 10.47 ± 1.05 to 1.68 ± 0.55 (p < 0.05). Transcription of the studied receptors in the pathological tissue depended on the stage of the disease: in relapsing patients expression of estradiol receptors underwent some changes, while expression profile of progesterone receptors remained unchanged. Sensitivity of endometrial tissue to gestogens is clinically important and serves as a basis for a successful hormone-based relapse prevention.

Keywords: ovarian endometriosis, PCR, estradiol receptors, progesterone receptors