2018 / 02

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DOI: 10.24075/brsmu.2018.021

ORIGINAL RESEARCH

Cas13a: purification and use for detection of viral RNA

Savinova AS1,2, Koptev EYu1, Usachev EV1, Tkachuk AP1, Gushchin VA1,3
About authors

1 N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Moscow

2 Translational Biomedicine Laboratory,
N. F. Gamaleya Federal Research Centre for Epidemiology and Microbiology, Moscow

3 Department of Virology, Faculty of Biology,
Lomonosov Moscow State University, Moscow

Correspondence should be addressed: Vladimir A. Gushchin
Gamalei 18, Moscow, 123098; moc.liamg@adainawow; gro.ayelamag@nihchsug.a.rimidalv

Received: 2018-05-31 Accepted: 2018-06-07 Published online: 2018-07-01
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Fig. 1. SDS-PAGE of recombinant LwCas13a. Fraction analysis after IMAC: 1 — total protein; 2 — flow through; 3–9 — eluted fractions; M — molecular weight markers
Fig. 2. Fluorescence kinetics during incubation of different RNAseAlert
Fig. 3. Fluorescence kinetics during the study of non-specific RNAse activities of LwCas13a
Fig. 4. Fluorescence kinetics at different concentrations of target RNA
Fig. 5 Fluorescence kinetics at small concentrations of target RNA of the influenza B virus