DOI: 10.24075/brsmu.2018.052

ORIGINAL RESEARCH

The efficacy of CRISPR-Cas9-mediated induction of the CCR5delta32 mutation in the human embryo

Kodyleva TA1, Kirillova AO1, Tyschik EA1, Makarov VV2, Khromov AV2, Guschin VA2, Abubakirov AN1, Rebrikov DV1,3, Sukhikh GT1
About authors

1 Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow

2 Lomonosov Moscow State University, Moscow, Russia

3 Pirogov Russian National Research Medical University, Moscow, Russia

Correspondence should be addressed: Denis V. Rebrikov
Ostrovityanova 1, Moscow, 117997; moc.liamg@vokirberd

Received: 2018-09-26 Accepted: 2018-10-09 Published online: 2018-10-16
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Fig. 1. gRNAs layout inside the human CCR5 gene
Fig. 2. Examples of real-time PCR curves for different genotypes: A — two control embryos, B — two mosaic embryos with about 3% of WT CCR5, C — two CCR5delta32 homozygous embryos
Table 1. gRNAs layout inside the human CCR5 gene
Table 2. Real-time PCR Cp values for each embryo
Note: no PCR products