2018 / 04

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DOI: 10.24075/brsmu.2018.049

METHOD

High-performance aerosol sampler with liquid phase recirculation and pre-concentration of particles

Akmalov AE1, Kotkovskii GE1, Stolyarov SV1, Verdiev BI2, Ovchinnikov RS2, Pochtovyy AA2, Tkachuk AP2, Chistyakov AA1
About authors

1 National Research Nuclear University MEPhI, Moscow

2 N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Moscow

About paper

Funding: this work was supported by the Federal Target Program The National System for Chemical and Biological Security of the Russian Federation (2015- 2020), the state contract No. K-27-НИР/148-2 signed by the Ministry of Healthcare of the Russian Federation and the National Research Nuclear University MEPhI.

Received: 2018-07-27 Accepted: 2018-08-23 Published online: 2018-10-05
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Fig. 1. The schematic of the virtual impactor (cross-section). W is the width of the inlet nozzle; D is the width of the outlet nozzle; S is the distance between the two nozzles; q1 is the incoming air flow; q4 is the exiting air flow containing concentrated aerosol particles; q2 and q3 represent the discarded flow
Fig. 2. The schematic of a liquid-phase cyclone concentrator
Fig. 3. Dependency of particle collection efficiency on aerosol particle size at various inlet nozzle widths W (Fig. 1) of the virtual impactor. The outlet width is 0.07 cm
Fig. 4. Dependency of liquid collection efficiency on the aerosol particle size at various distances S between the inlet and outlet nozzles (Fig. 1) of the virtual impactor. The width of the inlet and outlet is 0.07 cm
Fig. 5. Dependency of liquid collection efficiency on the corner radius R of the inlet nozzle. The width of the inlet and outlet nozzles is 0.07
Fig. 6. The Cyclon-Bio device assembled. On the left: the impactor. On the right: the cyclone concentrator
Fig. 7. Overnight culture of the samples collected at Novokosino metro station during the morning rush hour and plated onto blood agar. On the left: sample A123 collected by the SASS sampler. On the right: sample A223, Cyclon sampler (MEPhI)
Table 1. Test of the sampler performance using a test formulation (Central Research Institute of the Ministry of Defense of the Russian Federation)
Table 2. Testing antibiotic susceptibility of the collected strains