ORIGINAL RESEARCH

Synthesis of Au (III) polyacrylates and study of their tumoricidal activity

Shibaeva AV1, Pozdniakova NV2, Spiridonov VV3, Smirnova MS4, Korman DB1, Ostrovskaya LA1, Abzaeva KA5, Belyakova AV1,6, Biryukova YuK1,6, Zylkova MV1, Ivanov KP7, Shevelev AB4, Kuzmin VA1
About authors

1 Emanuel Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow, Russia

2 Blokhin National Medical Research Center of Oncology, Moscow

3 Lomonosov Moscow State University, Moscow

4 Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia

5 Favorsky Irkutsk Institute of Chemistry, Irkutsk

6 Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, Moscow

7 National Medical Research Center for Cardiology, Moscow, Russia

Correspondence should be addressed: Alexei B. Shevelev
Gubkina 3, Moscow, 119991; moc.liamtoh@a_levehs

About paper

Funding: the study was supported by the Ministry of Science and Higher Education of the Russian Federation (Subsidy Contract 14.607.21.0199 dated 26.09.2017, Project ID RFMEFI60717X0199). Spectroscopy was performed at the Shared Research Facility of Favorsky Irkutsk Institute of Chemistry, RAS.

Received: 2018-09-28 Accepted: 2018-10-26 Published online: 2018-12-16
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Aurumacryl is an incomplete metal salt of poly(acrylic acid) that exhibits hemostatic activity and inhibits the growth of transplantable carcinomas in vivo. The samples of aurumacryl synthesized following the original technique are insufficiently soluble, which complicates the study of the mechanisms involved in their synthesis and underlying their cytotoxic effect. The aim of this work was to study the impact of the following factors on aurumacryl properties: the molecular weight of the polyacrylate polymer in a range between 2 and 1,000 kDa, the presence of a counterion H+ or Na+, the molar ratio of AuCl to the polyacrylate polymer (1 : 5 and 1 : 10), the total concentration of the polyacrylate polymer during synthesis (0.1 and 3%), and the type of drying (lyophilization). By comparing the cytotoxicity of aurumacryl samples with significantly different molar ratio of gold ions to the polyacrylate polymer against human breast carcinoma cells (MCF-7), we established that the proportion of the polymer and its molecular weight in the sample do not affect the biological properties of the synthesized substance. Using UV spectroscopy, we revealed that the concentration of Au (III) ions in aurumacryl determines its cytotoxicity.

Keywords: aurumacryl, polyacrylate, chloroauric acid tetrahydrate, antitumor therapy, cytotoxicity, MCF-7

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