ISSN Print 2500–1094
ISSN Online 2542–1204
Bulletin of RSMU
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Nanostructured photosensitizer based on a tetracationic derivative of bacteriochlorin for antibacterial photodynamic therapy
About authors
1 Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow
2 Department of laser micro-, nano- and biotechnologies, Institute of engineering physics for biomedicine, National Research Nuclear University "MEPHI", Moscow
3 Laboratory of gene engineering of pathogenic microorganisms, N.F. Gamaleya National Research Center of Epidemiology and Microbiology, Moscow
4 Organic Intermediates and Dyes Institute, Moscow
Correspondеnce should be adressed: Gennady A. Meerovich
Vavilova, 38, Moscow, 119991;
Received: 2018-08-31
Accepted: 2018-09-27
Published online: 2018-12-31
|
Making antibacterial PDT more effective is a task that calls for the development of photosensitizers (PS) based on polycationic synthetic bacteriochlorins and subsequent analysis of properties of such photosensitizers. This study aimed to explore photophysical and antibacterial properties of the nanostructured PS based on 3-Py4BSHp4Br4, tetracationic amphiphilic derivative of synthetic bacteriochlorin. The PS was solubilized in a 4% Kolliphor ELP to obtain its nanostructured dispersion. We researched the absorption and fluorescence spectra intensity and profiles, studying concentrations from 0.001 to 0.2 mM, and found that the aggregation level of the PS in question is low throughout the range investigated while the S. aureus (gram-positive) and P. aeruginosa and K. pneumoniae (gram-negative) PD inactivation effectiveness is high.
Keywords: photosensitizer, cationic bacteriochlorin, aggregation, nanostructured dispersion, fluorescence, antibacterial photodynamic therapy