BIOMEDICAL JOURNAL OF PIROGOV RNRMU (MOSCOW, RUSSIA)
Temporal dynamics of cytokines in the blood of rats with experimentally induced autoimmune encephalomyelitis
In this work we explore the temporal dynamics of cytokines in Dark Agouti rats with experimentally induced autoimmune encephalomyelitis (EAE). The main group consisted of 11 animals who were injected with 100 μl (per leg) of spinal cord homogenate obtained from random-bred rats and combined with incomplete Freund’s adjuvant to the hind footpads. The control group included 7 animals who received 100 μl of normal saline mixed with incomplete Freund’s adjuvant. Blood samples (500 μl) were collected daily, starting from day 1 through day 7. We ran a Bio-Plex-based multiplex cytokine assay on the samples using the Bio-Plex Pro Rat Cytokine 24-plex Assay kit. EAE in rats was shown to simulate progression of multiple sclerosis in humans in terms of temporal dynamics of lymphoproliferative and hematopoietic factors IL-1b, IL-2, IL-4, IL-5, IL-6, and IL-7. The studied model satisfactory imitates the dynamics of factors stimulating migration of lymphocytes, monocytes and other immune cells, including IL-17, RANTES (CCL-5) and MCP-1 (CCL-2) but excluding GRO/KC (CXCL1), which shows a different dynamics. The model also resembles patterns of human multiple sclerosis in terms of factors affecting cytotoxic and apoptotic reactions, including IFNγ, IL-6 and IL-17, but excluding TNFα.
Keywords: multiple sclerosis, immunization, experimental autoimmune encephalomyelitis, myelin, multiplex cytokine assay