ISSN Print 2500–1094
ISSN Online 2542–1204
Bulletin of RSMU
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
The search and analysis of a CRISPR-Cas system in Escherichia coli HS with subsequent scanning for the corresponding phage races based on the spacers of the detected CRIPSR array using bioinformatic methods
About authors
1 Scientific Center for Family Health and Human Reproduction Problems, Irkutsk
2 Research Institute for Biomedical Technologies of Irkutsk State Medical University, Irkutsk
Correspondence should be addressed: Elena Ivanova
Timiryazeva 16, Irkutsk, 66400; moc.liamg@mei.avonavi
Received: 2018-03-15
Accepted: 2018-03-24
Published online: 2018-06-27
|
Fig. 1. Cas-genes (A) and their location in the genome (B) of E. coli HS (CP000802) detected by MacSyFinder and visualized in MacSyView
Fig. 2. Structural and functional characteristics of Сas proteins of E. coli HS (CP000802) obtained in MacSyFinder
Fig. 3. The consensus view of the alternating repeats in the genome of E. coli HS (CP000802) generated in CRISPI: a CRISPR Interactive database. The size of nucleotide letter codes shows a degree of nucleotide variability in the repeat: the taller the letter, the more variable the nucleotide
Fig. 4. Location of cas-genes and the CRISPR array in the genome of E. coli HS (CP000802)
Table 1. The list of nucleotide sequences in the CRIPSR array: spacers separated by repeat units detected in CRISPI: a CRISPR Interactive database in the genome of E. coli HS (CP000802)
Table 2. Spectrum of the phage races revealed by the complementary structures of the spacer sequences of the CRISPR cassette of E. coli HS strain (No. CP000802)
