2023 / 06

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DOI: 10.24075/brsmu.2023.049

ORIGINAL RESEARCH

Relationship between pro-inflammatory cytokine levels and blood bacterial DNA composition in obese children

Roumiantsev SA1,2,3, Kirilina IV1,2,3, Gaponov AM2, Khusnutdinova DR4, Grigoryeva TV4, Teplyakova ED5, Makarov VV6, Yudin SM6, Shestopalov AV1,2,3
About authors

1 Pirogov Russian National Research Medical University, Moscow, Russia

2 Center for Digital and Translational Biomedicine, Center for Molecular Health, Moscow, Russia

3 The National Medical Research Center for Endocrinology, Moscow, Russia

4 Kazan (Volga Region) Federal University, Kazan, Russia

5 Rostov State Medical University, Rostov-on-Don, Russia

6 Center for Strategic Planning and Management of Biomedical Health Risks, Moscow, Russia

Correspondence should be addressed: Irina V. Kirilina
Ostrovityanova, 1, Moscow, 117997, Russia; ur.kb@aniri-anilirik

About paper

Funding: the work was done in the context of agreement #0373100122119000041 under the Project "Compilation of a bank of blood serum and faecal samples from healthy donors and patients with obesity, metabolic syndrome, type II diabetes mellitus, disrupted intestinal mucosal barrier, with the aim of identifying candidate species-specific mediators of the human microbiota quorum sensing systems modulating endocrine and metabolic functions of adipose tissue."

Author contribution: Shestopalov AV, Roumiantsev SA — idea of the study, experiment planning, manuscript writing, editing; Yudin SM, Makarov VV — idea of the study, editing; Gaponov AM — idea, experiment planning, manuscript editing; Kirilina IV — idea, experiment planning, data processing, manuscript writing, editing; Grigoryeva TV — planning, data collection, data processing, editing; Teplyakova ED — experiment planning, manuscript editing; Khusnutdinova DR — data collection.

Compliance with ethical standards: the study was approved by the Local Ethics Committee of N.I. Pirogov Russian National Research Medical University (Minutes #186 of June 26, 2019) and Local Ethics Committee of Rostov State Medical University (Minutes #20/19 of December 12, 2019). To be included in the study, all patients and their parents mandatorily signed voluntary informed consent forms.

Received: 2023-10-13 Accepted: 2023-11-25 Published online: 2023-12-23
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  1. Shin JJ, Lee EK, Park TJ, Kim W. Damage associated molecular patterns and their pathological relevance in diabetes mellitus. Ageing Research Reviews. 2015; 24 (Pt A): 66–76. Epub 2015 Jul 18.
  2. Land WG. The role of damage-associated molecular patterns in human diseases: part I — promoting inflammation and immunity. Sultan Qaboos University Medical Journal. 2015; 15: 9–21.
  3. Ballak D, van Asseldonk E, van Diepen J, et al. TLR-3 is present in human adipocytes, but its signalling is not required for obesityinduced inflammation in adipose tissue in vivo. PLоS ONE. 2015; 10 (4): e0123152.
  4. Yu L, Li Y, Du C, Zhao W, Zhang H, Yang Y, et al. Pattern recognition receptor-mediated chronic inflammation in the development and progression of obesity-related metabolic diseases. Hindawi Mediators of Inflamm. 2019 Sep; 2019: 5271295. PubMed PMID: 31582899. PubMed Central PMCID: PMC6754942.
  5. Christodoulides C, Vidal-Puig A. PPARs and adipocytes function. Mol Cell Endocrinol. 2010 Apr; 318 (1–2): 61–8. Epub 2009 Sep 20.
  6. Caporaso JG, Kuczynski J, Stombaugh J, et al. QIIME allows analysis of high-throughput community sequencing data. Nature Methods. 2010 May; 7 (5): 335–6. Epub 2010 Apr 11.
  7. DeSantis T, Hugenholtz P, Larsen N, et al. Greengenes, a ChimeraChecked 16S rRNA Gene Database and Workbench Compatible with ARB. Appl Environ Microbiol. 2006; 72 (7): 5069–72.
  8. Kirilina IV, Shestopalov AV, Gaponov AM, Kamaldinova DR, Khusnutdinova DR, Grigorieva TV, et al. Features of the blood microbiome in obese children. Pediatria n.a. G. N. Speransky. 2022; 101 (5): 15–22. Russian.
  9. Maxwell JR, Zhang Y, Brown WA, Smith CL, Byrne FR, Fiorino M et al. Differential roles for Interleukin-23 and Interleukin-17 in intestinal immunoregulation. Immunity. 2015; 43: 739–50.
  10. Brevi A, Cogrossi LL, Grazi G, Masciovecchio D, Impellizzieri D, Lacanfora L, et al. Much more than IL-17A: cytokines of the IL-17 family between microbiota and cancer. Mini rewiew article Front. Immunol. Sec. Cancer Immunity and Immunotherapy. 2020 Nov; 11: 565470.
  11. Bi Y, Li C, Liu L, Zhou J, Li Z, Deng H, et al. IL-17A-dependent gut microbiota is essential for regulating diet-induced disorders in mice. Science Bulletin. 2017; 62 (15): 1052–63.
  12. Shahi SK, Ghimire S, Jensen SN, Lehman P, Borcherding N, Gibson-Corley KN, et al. IL-17A controls CNS autoimmunity by regulating gut microbiota and inducing regulatory T cells bioRxiv. 2022 Apr; 04.22.489206.
  13. Chen K, Kolls JK. Interluekin-17A (IL17A). Gene. 2017; 30 (614): 8–14. Epub 2017 Jan 22.
  14. Wang X, Teng F, Kong Li, Jinming Yu. PD-L1 expression in human cancers and its association with clinical outcomes. Onco Targets Ther. 2016; 9: 5023–39.
  15. Wu Y, Chen W, Xu ZP, Gu W. PD-L1 distribution and perspective for cancer immunotherapy-blockade, knockdown, or inhibition. Front Immunol. 2019; 10: 2022.
  16. Jiang X, Wang J, Deng X, Xiong F, Ge J, Xiang B, et al. Role of the tumor microenvironment in PD-L1/PD-1-mediated tumor immune escape. Mol Cancer. 2019; 18 (1): 10.
  17. Dermani FK, Samadi P, Rahmani G, Kohlan AK, Najafi R. PD1/ PD-L1 immune checkpoint: Potential target for cancer therapy. J Cell Physiol. 2019; 234 (2): 1313–25.
  18. Ingram JR, Dougan M, Rashidian M, Knoll M, Keliher EJ, Garrett S, et al. PD-L1 is an activation-independent marker of brown adipocytes. Nature Communication. 2017 Sep 21; 8 (1): 647.
  19. Wu B, Chiang H-C, Sun X, Yuan B, Mitra P, Hu Y, et al. Genetic ablation of adipocyte PD-L1 reduces tumor growth but accentuates obesity-associated inflammation. J Immunother Cancer. 2020 Aug; 8 (2): e000964. PubMed PMID: 32817394. PubMed Central PMCID: PMC7437875.
  20. Fujii T, Nishiki E, Endo M, Yajima R, Katayama A, Oyama T. Implication of atypical supraclavicular F18-fluorodeoxyglucose uptake in patients with breast cancer: Relationship between brown adipose tissue and TILs, PD-L1. Posters A: Risk factors. 2020 Oct; (Suppl 1): S94.
  21. Gopalakrishnan V, Spencer CN, Nezi L, Reuben A, Andrews MC, Karpinets TV, et al. Gut microbiome modulates response to anti– PD-1 immunotherapy in melanoma patients. Science. 2018 Jan 5; 359 (6371): 97–103. Epub 2017 Nov 2.
  22. Plotnikova EY, Krasnov OA. Metabolic syndrome and intestinal microflora: what overall? Experimental and Clinical Gastroenterology. 2015; 112 (12): 64–73. Russian.
  23. Mouzaki M, Comelli EM, Arendt BM, Bonengel J, Fung SK, et al. Intestinal microbiota in patients with nonalcoholic fatty liver disease. Hepatology. 2013; 58: 120–7.
  24. Zhu L, Baker SS, Gill C, Liu W, Alkhouri R, et al. Characterization of gut microbiomes in nonalcoholic steatohepatitis (NASH) patients: A connection between endogenous alcohol and NASH. Hepatology. 2012; 57: 601–9.
  25. Loranskaya ID, Khalif IL, Boldyreva MN, Kupaeva VA. Characteristic of microbiome in inflammatory bowel disease. Experimental and Clinical Gastroenterology. 2018; 153 (5): 104–11. Russian.
  26. Shestopalov AV, Kolesnikova IM, Savchuk DV, Teplyakova ED, Shin VA, Grigoryeva TV, et al. Effect of the infant feeding type on gut microbiome taxonomy and levels of trefoil factors in children and adolescents. Russian Journal of Physiology. 2023; 109 (5): 656–72. Russian.