ISSN Print 2500–1094
ISSN Online 2542–1204
Bulletin of RSMU
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
METHOD
Tissue chemiluminescence as a method of evaluation of superoxide radical producing ability of mitochondria
About authors
Department of Medical Biophysics, Faculty of Fundamental Medicine,Lomonosov Moscow State University, Moscow, Russia
Correspondence should be addressed: Aishat Dzhatdoeva
Lomonosovskiy prospekt, d. 31, korp. 5, Moscow, Russia, 119192; moc.liamg@jdtahsya
About paper
Funding: this work was supported by the Russian Science Foundation (project no. 14-15-00375).
Acknowledgments: authors thank Aleksey Grishin, a student at the Department of Physiology, Lomonosov Moscow State University, for his help in choosing gas mixture components.
Received: 2015-10-01
Accepted: 2015-12-09
Published online: 2017-01-05
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Fig. 1. (A) Experimental setup scheme. 1, 2 — tanks for two different gas mixtures (oxygen and oxygen-free mixtures); 3 — gas mixture switch; 4 — tubes supplying gas mixture to the chemiluminometer cuvette; 5 — peristaltic pump feeding gas mixture to the system; 6 — SmartLum-100 chemiluminometer; 7 — computer. (B) Position of the test sample in the chemiluminometer
Fig. 2. (A) Change in pH and (B) development of lucigenin-enhanced chemiluminescence in one of the rat heart samples with aeration of samples with gas mixtures of different composition
1 — curve for exhaled air (O2 — 5 %, CO2 — 4,3 %, N2 — 74,0 %); 2 — curve for atmospheric air (O2 — 21,0 %, CO2 — 0,03 %, N2 — 78,0 %); 3 — curve for carbogen (O2 — 95,0 %, CO2 — 5,0 %). Arrow indicates start of aeration.
Fig. 3. Curves of lucigenin-enhanced chemiluminescence in one of the rat heart samples for different hypoxia models. (A) Model 1 (hypoxia cycle — 15 min). (B) Model 2 (hypoxia cycle - 150 min). (C) Model 3 (hypoxia cycle — 240 min)
Fig. 4. Development of lucigenin-enhanced chemiluminescence in mouse brain tissue sections containing the striatum (A) and substantia nigra (B)
1 — curve for control sample, 2 — curve for test sample.
Table 1. Influence of hypoxia duration on formation of superoxide anion radical in rat heart tissue, M ± m (n = 5, p < 0.05)
Table 2. Formation of superoxide anion radical in mouse brain tissue when modeling parkinsonism, M ± m (n = 5, * — p < 0.05)
