BIOMEDICAL JOURNAL OF PIROGOV RNRMU (MOSCOW, RUSSIA)
Issue 2, 2018
Сomplex genomes editing with CRISPR-Cas systems is the most promising approach to introduce exact changes in DNA in vivo, both in scientific applications and in medicine. The diversity of CRISPR-Cas systems (protecting the bacterium from a virus attack) allowed researchers to deduce a wide range of enzymes with various properties for the lab applications. However, the new genome editing systems investigation is unstoppable and in the near future we will see CRISPR-Cas systems with new characteristics of efficiency and accuracy.
Issue 3, 2017
Several thousand drugs for gene therapy are being tested clinically. Targets for them are, as a rule, protein-coding genes. But the accumulation of knowledge about RNA and the role of RNA of various types in cellular processes makes these molecules also promising targets. Alexandra Filatova (Research Centre of Medical Genetics, Moscow) et al. talk about long noncoding RNA; Yulia Mogulevtseva (RSAU–MAA named after K. A. Timiryazev) et al. give an example of the use of RNA interference in the treatment of psoriasis; Ivan Chicherin (Lomonosov Moscow State University) et al. talk about the possibility of creating a technology for editing mitochondrial DNA based on CRISPR/Cas9.
Issue 4, 2017
Noninvasiveness is an attractive advantage of any diagnostic technique, including liquid biopsy, a method for the analysis of cell-free DNA circulating in patient’s blood that holds special promise for cancer research. Maxim Filipenko of the Institute of Chemical Biology and Fundamental Medicine (SB RAS, Novosibirsk) reports achievements in this field. Ekaterina Sokolova of BIOCAD (Moscow) and her fellow researchers present a modified multiplex PCR technique developed to estimate the degree of cfDNA fragmentation. Elena Tyschik and her colleagues of Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology (Moscow) analyze the abundance of exRNA of VEGF isoforms in patients with breast cancer.
Issue 5, 2017
Our immune system is capable of “remembering” pathogens. This phenomenon is exploited to establish protection against infection by vaccination. Vladimir Guschin of Gamaleya Research Institute of Epidemiology and Microbiology (Moscow) et al. describe the contemporary approaches to studying population immunity, propose a system for serological surveillance in Russia and in the original research report the first results of the clinical trial of GamTBvac, a candidate recombinant vaccine against tuberculosis. Ekaterina Merzlyak of the Institute of Bioorganic Chemistry et al. study the repertoires of T-cell receptors in patients with autoimmune vasculitis following treatment with cyclophosphamide.
Issue 6, 2017Rehabilitation is one of the most crucial stages in the therapy of many diseases. The quality of the patient's further life largely depends on the quality of rehabilitation. The issue discusses rehabilitation in medicine in the context of sports injuries. Also, several important works in the field of oncology, immunology, physiology and hygiene were published.
Issue 1, 2018For almost 100 years now, mankind has been fighting incessantly against pathogenic bacteria with the help of more and more new antibiotics. But evolutionary flexibility allows pathogens to bypass our locks almost at the speed of the invention. One of the good alternatives to speeding up and reducing the cost of this arms race is the bacteriophage.