2017 / 04

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DOI: 10.24075/brsmu.2017-04-09

CLINICAL CASE

Identification of the atypical bacterial strain Streptococcus intermedius that caused brain abscess in the patient using Sanger sequencing of the 16S rRNA gene from the DNA extracted from a pus sample

Gordukova MA1, Divilina YuV1, Mishukova OV2, Galeeva EV1, Prodeus AP1, Filipenko ML2
About authors

1 Clinical Diagnostic Laboratory,
Speransky Children's Clinical Hospital No. 9, Moscow, Russia

2 Laboratory of Pharmacogenomics,
Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia

Correspondence should be addressed: Maria Gordukova
Shmitovsky proezd, d. 29, Moscow, Russia, 123317; ur.9-bkgd@avokudrog.am

About paper

All authors' contribution to this work is equal: selection and analysis of literature, research planning, data collection, analysis, and interpretation, drafting of a manuscript, editing.

Received: 2017-07-04 Accepted: 2017-08-04 Published online: 2017-10-31
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  1. Lehmann LE, Hunfeld KP, Emrich T, Haberhausen G, Wissing H, Hoeft A et al. A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med Microbiol Immunol. 2008 Sep; 197 (3): 313–24. DOI: 10.1007/s00430-007-0063-0.
  2. Bruce IJ. Nucleic acid amplification mediated microbial identification. Sci Prog. 1993–1994; 77 (Pt 3–4): 183–206.
  3. Woese CR. Bacterial evolution. Microbiol Rev. 1987 Jun; 51 (2): 221–71.
  4. Relman DA. The search for unrecognized pathogens. Science. 1999 May 21; 284 (5418): 1308–10.
  5. Schmidt TM, Relman DA. Phylogenetic identification of uncultured pathogens using ribosomal RNA sequences. Methods Enzymol. 1994; 235: 205–22.
  6. Brouqui P, Raoult D. Endocarditis due to rare and fastidious bacteria. Clin Microbiol Rev. 2001 Jan; 14 (1): 177–207. DOI: 10.1128/CMR.14.1.177-207.2001.
  7. Harris KA, Fidler KJ, Hartley JC, Vogt J, Klein NJ, Monsell F et al. Unique case of Helicobacter sp. osteomyelitis in an immunocompetent child diagnosed by broad-range 16S PCR. J Clin Microbiol. 2002 Aug; 40 (8): 3100–3.
  8. Harris KA, Hartley JC. Development of broad-range 16S rDNA PCR for use in the routine diagnostic clinical microbiology service. J Med Microbiol. 2003 Aug; 52 (Pt 8): 685–91. DOI: 10.1099/jmm.0.05213-0.
  9. Xia LP, Bian LY, Xu M, Liu Y, Tang AL, Ye WQ. 16S rRNA gene sequencing is a non-culture method of defining the specific bacterial etiology of ventilator-associated pneumonia. Int J Clin Exp Med. 2015 Oct 15; 8 (10): 18560–70.
  10. Daroy ML, Lopez JS, Torres BC, Loy MJ, Tuano PM, Matias RR. Identification of unknown ocular pathogens in clinically suspected eye infections using ribosomal RNA gene sequence analysis. Clin Microbiol Infect. 2011 May; 17 (5): 776–9. DOI: 10.1111/j,1469-0691.2010.03369.x.
  11. Mishra AK, Fournier PE. The role of Streptococcus intermedius in brain abscess. Eur J Clin Microbiol Infect Dis. 2013 Apr; 32 (4): 477–83. DOI: 10.1007/s10096-012-1782-8.
  12. Saito N, Hida A, Koide Y, Ooka T, Ichikawa Y, Shimizu J et al. Culture-negative brain abscess with Streptococcus intermedius infection with diagnosis established by direct nucleotide sequence analysis of the 16s ribosomal RNA gene. Intern Med. 2012; 51 (2): 211–6.