2017 / 06

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DOI: 10.24075/brsmu.2017-06-10

ORIGINAL RESEARCH

Analysis of the apoptotic effect of ultrahigh gamma dose rates on human peripheral blood lymphocytes in vitro

Grabovsky EV1, Oleynik GM1, Krastelev EG2, Smirnov VP2,3, Khmelevsky EV4, Bozhenko VK5, Shishkin AM5, Ivanov AV5, Kulinich TM5
About authors

1 Troitsk Institute for Innovation and Fusion Research (TRINITI),
Rosatom State Atomic Energy Corporation, Troitsk, Moscow, Russia

2 Joint Institute for High Temperatures, Russian Academy of Sciences, Moscow, Russia

3 Research Institute of Technical Physics and Automation,
Rosatom State Atomic Energy Corporation, Moscow, Russia

4 Hertzen Moscow Cancer Research Institute (affiliated branch of the National Medical Research Radiology Center), Moscow, Russia

5 Russian Scientific Center of Roentgenoradiology, Moscow

Correspondence should be addressed: Vladimir K. Bojenko
ul. Profsoyuznaya, d. 86, Moscow, Russia, 117997; ur.liam@oknejobv

About paper

Funding: this work was supported by Rosatom and the Russian Science Foundation (Grant no. 15-10355).

All authors' contribution to this work is equal: selection and analysis of literature, research planning, data collection, analysis, and interpretation, drafting of a manuscript, editing.

Received: 2017-11-22 Accepted: 2017-12-05 Published online: 2018-01-25
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Fig. 1. Schematic of generation and delivery of the electromagnetic pulse by Angara-5-1 generator to the load in the vacuum chamber. PFL — double water pulse- forming line; TL — water transmission line; VWI — vacuum-water interface; VTL — magnetically insulated vacuum transmission line; VC — vacuum chamber with electromagnetic flux concentrator
Fig. 2. Position of the samples for irradiation by Mir-M (A, B), Angara-5-1 (C) and Rokus-AM (D)
Fig. 3. Levels of apoptosis in the suspensions of peripheral blood lymphocytes irradiated by Rokus-AM and Angara-5-1 with doses from 0 to 5 Gy
Fig. 4. Comparison of the effects induced by electron beams generated by high-current nanosecond electron accelerator Mir-M and gamma rays produced by Rokus-AM on human peripheral blood lymphocytes in the dose range from 0 to 6 Gy. (A) Early apoptosis (annexin V+/PI–); (B) late apoptosis (annexin V+/PI+); (C) necrosis (annexin V–/PI+); (D) total cell death. PI — propidium iodide. Positive and negative staining is represented by + and – respectively