2019 / 01

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DOI: 10.24075/brsmu.2019.009

ORIGINAL RESEARCH

Evaluation of the ejaculate microbiota by real-time PCR and culture-based technique

Voroshilina ES1,2, Zornikov DL, Panacheva EA2
About authors

1 Ural State Medical University, Yekaterinburg, Russia

2 Medical Center “Garmonia”, Yekaterinburg, Russia

Correspondence should be addressed: Еkaterina S. Voroshilina
Furmanova 30, Yekaterinburg, 620142; moc.liamg@anilihsorov

About paper

Acknowledgments: the authors would like to thank V. N. Khayutin, director of Garmonia Medical Center, for allowing to conduct the study in the clinic's laboratory department.

Author contribution: Voroshilina ES — organization of the study, conducting tests, data analysis, article authoring; Zornikov DL — data analysis, statistical processing, article authoring; Panacheva EA — literature review, clinical data collection, statistical analysis, article authoring.

Received: 2019-02-11 Accepted: 2019-03-28 Published online: 2019-03-11
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Table 1. Semen microbiota variants, RT PCR data (n = 86)
Note: 1 — this microbiota type was suggested due to the specifics of the Androflor test (Enterobacteriaceae spp. / Enterococcus spp. are detected in a tube separately from other Gram-positive and Gram-negative facultative anaerobes without species identification); 2 — this variant was applied when a proportion of several detected groups of microorganisms slightly differed from each other; 3 — Fisher's test enabled calculation of significance of the differences.
Table 2. Comparison of the predominant groups of microorganisms according to culture-based technique and RT PCR results (n = 86)