ISSN Print 2500–1094
ISSN Online 2542–1204
Bulletin of RSMU
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Humoral response to Epstein-Barr viral infection in patients with allergies
About authors
1 Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia
2 Institute of Continuing Vocational Education, Federal Medical Biological Agency, Moscow, Russia
3 Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
Correspondence should be addressed: Elena V. Svirshchevskaya
Miklouho-Maclay 16/10, Moscow, 117997; ur.hcbi.liam@rivse
About paper
Author contribution: Svirshchevskaya EV measured IgE titers against allergens in the sera of allergic individuals and healthy donors, processed the obtained data and participated in the writing of this article; Khlgatian SV selected sera samples for the study and conducted RIDA assays; Fattakhova GV and Chudakov DB measured IgE titers against allergens in the sera of patients with allergies and healthy donors; Matushevskaya EV collected sera samples from patients with allergies, participated in the discussion of the study results and in the writing of this article; Simonova MA and Ryazantsev DYu performed iPCR; Ryazantsev DYu expressed recombinant proteins of EBV, A. alternata and D. farinaе; Chudakov DB synthesized, purified and characterized the sufficient amount of recombinant proteins for the study; Zavriev SK optimized PCR and participated in the discussion of the study results.
Received: 2018-09-12
Accepted: 2019-02-15
Published online: 2019-03-01
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Fig. 1. IgE-mediated response in patients with allergies measured by RIDA. IgE levels in the serum samples of patients allergic to house dust mites (A) and А. alternata (B) were interpreted as classes 0–6 proportional to IgE titers
Fig. 2. Analysis of rEBNA1-recognizing immunoglobulins present in the sera of healthy donors and patients with allergies. Analysis of IgA1, IgA2, IgM (A), IgG1, IgG2, IgG3, and IgG4 (B) in the pooled sera of 10 donors and 10 allergic patients. The figures are presented as mean values ± a standard deviation. Statistically significant differences (p < 0.05) are marked with a vertical line with a cross-bar
Fig. 3. Analysis of rEBNA1-specific IgG1 in the sera of healthy donors and patients with allergies. A. Analysis of IgG1 titers in individual sera of patients allergic to A. alternata (n = 15), house dust mites (n = 9) and healthy donors (n = 38). Vertical lines with cross-bars indicate mean values ± a standard deviation. B. Relationship between rEBNA1-specific IgG1 titers and age in the individual samples of sera collected from allergic patients (n = 53) and healthy donors (n = 34). Power approximation is marked with lines and t-test probability is also shown
Fig. 4. Analysis of rEBNA1-specific IgG1 present in the sera of healthy donors and patients with allergies. The proportion (%) of patients and healthy donors with different IgG1 titers (A) and the mean titers in these groups (B)
Fig. 5. Analysis of rEBNA1-specific IgA1 and IgM in the sera of healthy donors and patients with allergies. Distribution of IgA1 (A) and (B) titers in the individual serum samples of patients allergic to A. alternata (n = 11), house dust mites (n = 21) and healthy donors (n = 23) depending on donors’ age. Distribution of rEBNA1-specific IgA1 (C) and IgM (D) in the sera of healthy donors and patients with allergies. IgM data are given for the sera with low (grey circles) and high (black circles) IgM titers. Vertical lines with cross-bars show mean values ± a standard deviation. Significant differences (p < 0.05) are marked with horizontal bars
Table. Comparison of iPCR and ELISA steps
Note: *аIgG1-bio represents any biotinylated antibody to any class or subclass of antibodies (G, A, E); **HP is horseradish peroxidase.
