2020 / 02

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DOI: 10.24075/brsmu.2020.015

ORIGINAL RESEARCH

Characterization of the genotype and the phenotype of nontoxigenic strains of Corynebacterium diphtheriae subsp. lausannense isolated in Russian residents

Borisova OYu1,2, Chaplin AV1,2, Gadua NT1, Pimenova AS1, Alexeeva IN3, Rakitsky GF3, Afanas'ev SS1, Donskikh EE2, Kafarskaya LI2
About authors

1 G. N. Gabrichevsky Research Institute for Epidemiology and Microbiology, Moscow, Russia

2 Pirogov Russian National Research Medical University, Moscow, Russia

3 Regional Clinical Psychiatric Hospital, Khabarovsk, Russia

Correspondence should be addressed: Olga Yu. Borisova
Admirala Makarova, 10, Moscow, 125212; ur.liam@avosirobglo

About paper

Compliance with ethical standards: the study was approved by the Ethics Committee of G. N. Gabrichevsky Research Institute for Epidemiology and Microbiology. Informed consent was obtained from all participants.

Author contribution: Borisova OYu carried out molecular genetic tests, analyzed the literature and the obtained data, contributed to manuscript preparation; Chaplin AV performed phylogenetic analysis, analyzed the experimental data and contributed to manuscript preparation; Gadua NT, Pimenova AS carried out microbiological tests and contributed to manuscript preparation; Alexeeva IN, Rakitsky GF examined the patients on admission and contributed to manuscript preparation; Afanasiev SS conducted molecular genetic tests and contributed to manuscript preparation; Donskikh EE аnalyzed the literature and the experimental data and contributed to manuscript preparation; Kafarskaya LI analyzed the experimental data and contributed to manuscript preparation.

Received: 2020-02-09 Accepted: 2020-03-28 Published online: 2020-03-18
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Fig. 1. A phylogenetic tree for С. diphtheriae strains with publicly available sequenced genomes. The length of the branches and the genome of C. ulcerans BR-AD22 used for rooting are not shown in the figure. The numbers on the branches represent bootstrap values. Strains of C. diphtheriae subsp. lausannense are shown in gray. The numbers in brackets refer to the predicted sequence types and follow the PubMLST nomenclature (dash marks represent yet undescribed sequence types)
Fig. 2. The clonal complex reconstructed from PubMLST data. The complex comprises lausannense strains with publicly available sequenced genomes
Fig. 3. Gel electrophoresis of PCR products with the following primers: ptsI_F — ptsI_R and narG_F — narG_R. М is a GeneRuler 100 bp DNA Ladder (Thermo Fisher Scientific; USA) (example)
Fig 4. Saccharolytic acivity of C. diphtheriae subsp. lausannense strains: the ability to ferment N-acetylglucosamine. The crimson color of the medium means the test results are positive. 1, 2 are isolated strains of C. diphtheriae subsp. lausannense; 3 is a strain of gravis C. diphtheriae subsp. diphtheriae; 4 is a strain of mitis C. diphtheriae subsp. diphtheriae; 5 — negative control
Table. Characteristics of the analyzed C. diphtheriae cultures