2020 / 04

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DOI: 10.24075/brsmu.2020.044

ORIGINAL RESEARCH

Gut microbiota assessment in Moscow long-livers using next generation sequencing

Kashtanova DA1,2, Klimenko NS3, Strazhesko ID1, Tkacheva ON1, Starikova EV4, Glushchenko OE4, Gudkov EA4, Ilina EN4
About authors

1 Pirogov Russian National Research Medical University, Moscow, Russia

2 Center for Strategic Planning and Management of Medical and Biological Health Risks, Moscow, Russia

3 Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia

4 Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, Russia

Correspondence should be addressed: Daria A. Kashtanova
1-ya Leonova, 16, 129226; moc.liamg@avonathsak.rd

About paper

Funding: the study was supported by the Russian Foundation for Basic Research (Grant 19-34-80033).

Compliance with ethical standards: the study was approved by the Ethics Committee of Pirogov Russian National Research Medical University (Protocol № 2 dated March 18, 2016). Informed consent was obtained from all study participants.

Author contribution: Kashtanova DA — study design, participant recruitment, data interpretation, manuscript preparation; Klimenko NS — bioinformatic analysis, data interpretation, manuscript preparation; Strazhesko ID — study concept, manuscript revision; Tkacheva ON — study concept and design; Starikova EV — gut microbiota profiling, manuscript revision; Glushchenko OE, Gudkov DA — gut microbiota profiling; Ilina EN — final revision of the manuscript.

Received: 2020-07-01 Accepted: 2020-07-15 Published online: 2020-07-27
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Fig. 1. Phylum distribution of gut microorganisms in the groups of centenarians and relatively healthy younger participants
Fig. 2. A multidimensional scaling plot of gut microbiota samples obtained from centenarians (red dots) and relatively healthy individuals (blue dots).
Fig. 3. Metabolic pathways for butyric acid synthesis in the groups (the mean prevalence of the pathways is plotted on the Y axis)
Table 1. Clinical characteristics of the centenarian group
Note: HSCRP — high-sensitivity С-reactive protein; CIMT — carotid intima-media thickness; IQR — interquartile range; IADL — Instrumental Activities of Daily Living; MMSE — Mini-Mental State Examination; MNA — Mini Nutritional Assessment; МОСА — Montreal Cognitive Assessment.
Table 2. Microorganisms found to be more abundant in the gut microbiota of centenarians
Table 3. Microorganisms found to be more abundant in the gut microbiota of relatively healthy participants
Table 4. Associations between MNA scores and the prevalence of metabolic pathways for vitamin synthesis by the gut microbiota