2017 / 06

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DOI: 10.24075/brsmu.2017-06-11

ORIGINAL RESEARCH

The choice of anesthetic type and conditions for 2,3,5-triphenyltetrazolium chloride staining of brain slices is important in the assessment of ischemic injury in rats in the early stages of pathology

Bilan DS1,2, Kelmanson IV1, Belousov VV1,2
About authors

1 Laboratory for Molecular Technologies,
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia

2 Department of Brain-Computer Interfaces, Research Institute for Translational Medicine,
Pirogov Russian National Research Medical University, Moscow, Russia

Correspondence should be addressed: Vsevolod Belousov
ul. Miklukho-Maklaya, d. 16/10, Moscow, Russia, 117997; ur.hcbi@vosuoleb

About paper

Funding: this work was supported by the Russian Science Foundation (Grant 17-15-01175).

All authors' contribution to this work is equal: selection and analysis of literature, research planning, data collection, analysis, and interpretation, drafting of a manuscript, editing.

Received: 2017-11-29 Accepted: 2017-12-10 Published online: 2018-01-25
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Fig. 1. Effects of different temperatures and duration of incubation of rat brain slices in 1 % TTC solution on visualization of ischemic injury 5 hours after the occlusion. The pictures show brain slices obtained from a Wistar rat with the occluded middle cerebral artery. One slice was stained at room temperature, another — at 37 °С. Samples were photographed at set time intervals. Anesthetic used: Zoletil/Rometar
Fig. 2. Effects of different anesthetics on the scope of ischemic injury in rats with the permanently occluded middle cerebral artery (5 hours after the occlusion). Brain slices were incubated under identical conditions in 1 % TTC solution for 30 min at 37 °С
Fig. 3. Brain slices of rats anesthetized with different drugs 24 hours after the induced permanent occlusion of the middle cerebral artery. The slices were incubated under identical conditions in 1 % TTC solution for 30 min at 37 °С