DOI: 10.24075/brsmu.2018.005


Observing the dynamics of the NAD+ to NADH ratio in Danio rerio embryo tissues using a genetically encoded biosensor

Bilan DS1,2, Shokhina AG1, Panova AS1,3, Belousov VV1,2
About authors

1 Laboratory for Molecular Technologies,
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia

2 Department of Brain-Computer Interfaces, Research Institute for Translational Medicine,
Pirogov Russian National Research Medical University, Moscow, Russia

3 Department of Biochemistry, Faculty of Biology,
Lomonosov Moscow State University, Moscow, Russia

Correspondence should be addressed: Vsevolod Belousov
ul. Miklukho-Maklaya, d. 16/10, Moscow, Russia, 117997; ur.hcbi@vosuoleb

About paper

Funding: this work was supported by the Russian Foundation for Basic Research (Grant 16-34-60175) and the Russian Federation Presidential Grant MK-6339.2016.4. Experiments were partially carried out using the equipment provided by the IBCH сore facility (CKP IBCH, supported by Russian Ministry of Education and Science, grant RFMEFI62117X0018).

Received: 2017-12-05 Accepted: 2017-12-25

The zebrafish Danio rerio is a popular model species for biomedical research focusing on modeling human diseases and screening of therapeutic drugs. Genetically encoded biosensors based on fluorescent proteins are widely used in many laboratories worldwide to study the biology of living systems of various complexity in vivo. The main advantage of these molecular tools is that they allow observing biological processes in intact systems in real time. In the present work we study the dynamics of the intracellular ratio of NAD+ to NADP in the yolk sac and tissues of D. rerio larvae using the SoNar biosensor following the injection of glycolisis end products, lactate and pyruvate.

Keywords: genetically encoded biosensors, Danio rerio, pyruvate, lactate