2017 / 01

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DOI: 10.24075/brsmu.2017-01-03

ORIGINAL RESEARCH

Polymorphism of the dtxR gene in the currently existing strains of Сorynebacterium diphtheriae

Chagina IA1, Perevarova YuS2, Perevarov VV2, Chaplin AV2, Borisova OYu1,2, Kafarskaya LI2, Afanas'ev SS1, Aleshkin VA1
About authors

1 G. N. Gabrichevsky Research Institute for Epidemiology and Microbiology, Moscow, Russia

2 Pirogov Russian National Research Medical University, Moscow, Russia

Correspondence should be addressed: Andrey Viktorovich Chaplin
ul. Ostrovityanova, d. 1, Moscow, Russia, 117997; moc.liamg@kidemoloko

About paper

Funding: this study was conducted as part of two projects: The Study of the Role of Microbial Communities in Human Oropharynx and Blood in Diphtheria, Pertussis and Other Infectious Inflammatory Diseases (Project ID А16-116021550311-2) and The Development of Molecular-Genetic Methods for Laboratory Diagnosis of Diphtheria and Pertussis (Project ID АААА-А16-116101810127-7) supported by the Sectoral Research Program of the Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (Problem-oriented Research in Epidemiological Surveillance of Infectious and Parasitic Diseases in 2016-2020).

Contribution of the authors to this work:Chagina IA — analysis of literature, data collection and interpretation, drafting of a manuscript; Perevarova YuS, Perevarov VV — analysis of literature, data analysis and interpretation, drafting of a manuscript; Chaplin AV — research planning, data analysis and interpretation, drafting of a manuscript; Borisova OYu — research planning, data collection and interpretation, drafting of a manuscript; Kafarskaya LI — data interpretation, drafting of a manuscript; Afanas’ev SS, Aleshkin VA — research planning, drafting of a manuscript. All authors participated in editing of the manuscript.

Received: 2017-02-02 Accepted: 2017-02-18 Published online: 2017-03-11
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  1. Trost E, Blom J, Soares S de C, Huang IH, Al-Dilaimi A, Schröder J, et al. Pangenomic study of Corynebacterium diphtheriae that provides insights into the genomic diversity of pathogenic isolates from cases of classical diphtheria, endocarditis, and pneumonia. J Bacteriol. 2012 Jun; 194 (12): 3199–215.
  2. Deng Q, Barbieri JT. Molecular Mechanisms of the Cytotoxicity of ADP-Ribosylating Toxins. Annu Rev Microbiol. 2008; 62 (1): 271–88.
  3. Kombarova SU, Borisova OU, Melnikov VG, Naumov LS, Narvskaya OV, Mokrousov IV, et al. [Observation of circulation of Corynebacterium diphtheriae strains differing in toxigenicity character]. Meditsinskii al'manakh. 2009; (2): 108–11. Russian.
  4. Mel’nikov VG, Kombarova SIu, Borisova OIu, Volozhantsev NV, Verevkin VV, Volkovoj KI, et al. [Corynebacterium diphtheriae nontoxigenic strain carrying the gene of diphtheria toxin]. Zh Mikrobiol Epidemiol Immunobiol 2004 Jan–Feb; (1): 3–7. Russian.
  5. Merchant AT, Spatafora GA. A role for the DtxR family of metalloregulators in gram-positive pathogenesis. Mol Oral Microbiol. 2014 Feb; 29 (1): 1–10.
  6. Dinu S, Damian M, Badell E, Dragomirescu CC, Guiso N. New diphtheria toxin repressor types depicted in a Romanian collection of Corynebacterium diphtheriae isolates. J Basic Microbiol. 2014 Oct; 54 (10): 1136–9.
  7. Yellaboina S, Ranjan S, Chakhaiyar P, Hasnain SE, Ranjan A. Prediction of DtxR regulon: identification of binding sites and operons controlled by Diphtheria toxin repressor in Corynebacterium diphtheriae. BMC Microbiol. 2004 Sep 24; 4: 38.
  8. Agranoff DD, Krishna S. Metal ion homeostasis and intracellular parasitism. Mol Microbiol. 1998 May; 28 (3): 403–12.
  9. Valko M, Morris H, Cronin MT. Metals, Toxicity and Oxidative Stress. Curr Med Chem. 2005; 12 (10): 1161–208.
  10. Rolerson E, Swick A, Newlon L, Palmer C, Pan Y, Keeshan B, et al. The SloR/Dlg metalloregulator modulates Streptococcus mutans virulence gene expression. J Bacteriol. 2006 Jul; 188 (14): 5033–44.
  11. Reyes-Caballero H, Campanello GC, Giedroc DP. Metalloregulatory proteins: metal selectivity and allosteric switching. Biophys Chem. 2011 Jul; 156 (2–3): 103–14.
  12. Krebs JE, Goldstein ES, Kilpatrick ST. Lewin’s Genes XI. 11th ed. Burlington: Jones & Bartlett Learning; 2014.
  13. D’Aquino JA, Ringe D. Mechanism of metal ion activation of the diphtheria toxin repressor DtxR. AIP Conf Proc. 2006; 851: 196–8.
  14. Bhattacharya N, Yi M, Zhou HX, Logan TM. Backbone Dynamics in an Intramolecular Prolylpeptide-SH3 Complex from the Diphtheria Toxin Repressor, DtxR. J Mol Biol. 2007 Dec 7; 374 (4): 977–92.
  15. Nakao H, Mazurova IK, Glushkevich T, Popovic T. Analysis of heterogeneity of Corynebacterium diphtheriae toxin gene, tox, and its regulatory element, dtxR, by direct sequencing. Res Microbiol. 1997 Jan; 148 (1): 45–54.
  16. Marshall OJ. PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR. Bioinformatics. 2004 Oct 12; 20 (15): 2471–2.
  17. Bolt F, Cassiday P, Tondella ML, DeZoysa A, Efstratiou A, Sing A, et al. Multilocus Sequence Typing Identifies Evidence for Recombination and Two Distinct Lineages of Corynebacterium diphtheriae. J Clin Microbiol. 2010 Nov; 48 (11): 4177–85.
  18. Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004 Mar 19; 32 (5): 1792–7.
  19. Nei M, Gojobori T. Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Mol Biol Evol. 1986 Sep; 3 (5): 418–26.
  20. Patefield WM. Algorithm AS 159: An Efficient Method of Generating Random R × C Tables with Given Row and Column Totals. J R Stat Soc Ser C Appl Stat. 1981; 30 (1): 91–7.
  21. Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci U S A. 2004 Jul 27; 101 (30): 11030–5.
  22. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol. 2016 Jul; 33 (7): 1870–4.
  23. Borisova ОYu, Chagina IA, Chaplin AV, Kombarova SYu, Kafarskaya LI, Aleshkin VA. [Specificities of the structure of the tox gene encoding diphtheria toxin of Сorynebacterium diphtheriaе strains isolated in Russia in 2010–2015]. Infektsionnye bolezni. 2015; 13 (3): 12–7. Russian.
  24. Yang Z, Bielawski JP. Statistical methods for detecting molecular adaptation. Trends Ecol Evol. 2000 Dec 1;15(12):496–503.
  25. Kombarova SIu, Mazurova IK, Mel’nikov VG, Kostiu- kova NN, Volkovoj KI, Borisova OIu, et al. [Genetic structure of Corynebacterium diphtheriae strains isolated in Russia during epidemics of various intensity]. Zh Mikrobiol Epidemiol Immunobiol. 2001 May–Jun; (3): 3–8. Russian.
  26. Serwold-Davis TM, Groman N, Rabin M. Transformation of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum, and Escherichia coli with the C. diphtheriae plasmid pNG2. Proc Natl Acad Sci U S A. 1987 Jul; 84 (14): 4964–8.
  27. Sangal V, Blom J, Sutcliffe IC, von Hunolstein C, Burkovski A, Hoskisson PA. Adherence and invasive properties of Corynebacterium diphtheriae strains correlates with the predicted membrane-associated and secreted proteome. BMC Genomics. 2015 Oct 9; 16: 765.
  28. Drazek ES, Hammack CA, Schmitt MP. Corynebacterium diphtheriae genes required for acquisition of iron from haemin and haemoglobin are homologous to ABC haemin transporters. Mol Microbiol. 2000 Apr; 36 (1): 68–84.
  29. Sangal V, Burkovski A, Hunt AC, Edwards B, Blom J, Hoskisson PA. A lack of genetic basis for biovar differentiation in clinically important Corynebacterium diphtheriae from whole genome sequencing. Infect Genet Evol. 2014 Jan; 21: 54–7.
  30. Oram DM, Avdalovic A, Holmes RK. Construction and characterization of transposon insertion mutations in Corynebacterium diphtheriae that affect expression of the diphtheria toxin repressor (DtxR). J Bacteriol. 2002 Oct; 184 (20): 5723–32.
  31. Boyd JM, Hall KC, Murphy JR. DNA sequences and characterization of dtxR alleles from Corynebacterium diphtheriae PW8(-), 1030(-), and C7hm723(-). J Bacteriol. 1992 Feb; 174 (4): 1268–72.
  32. Muttaiyah S, Best EJ, Freeman JT, Taylor SL, Morris AJ, Roberts SA. Corynebacterium diphtheriae endocarditis: A case series and review of the treatment approach. Int J Infect Dis. 2011 Sep; 15 (9): e584–8.
  33. Barakett V, Morel G, Lesage D, Petit JC. Septic arthritis due to a nontoxigenic strain of Corynebacterium diphtheriae subspecies mitis. Clin Infect Dis. 1993 Sep; 17 (3): 520–1.
  34. Farfour E, Badell E, Zasada A, Hotzel H, Tomaso H, Guillot S, et al. Characterization and comparison of invasive Corynebacterium diphtheriae isolates from France and Poland. J Clin Microbiol. 2012 Jan; 50 (1): 173–5.
  35. Lake JA, Ehrhardt MJ, Suchi M, Chun RH, Willoughby RE. A Case of Necrotizing Epiglottitis Due to Nontoxigenic Corynebacterium diphtheriae. Pediatrics. 2015 Jul; 136 (1): e242–5.
  36. Wojewoda CM, Koval CE, Wilson DA, Chakos MH, Harrington SM. Bloodstream Infection Caused by Nontoxigenic Corynebacterium diphtheriae in an Immunocompromised Host in the United States. J Clin Microbiol. 2012 Jun; 50 (6): 2170–2.
  37. Pimenta FP, Matias GA, Pereira GA, Camello TC, Alves GB, Rosa AC, et al. A PCR for dtxR gene: Application to diagnosis of non-toxigenic and toxigenic Corynebacterium diphtheriae. Mol Cell Probes. 2008 Jun; 22 (3): 189–92.