2018 / 06

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DOI: 10.24075/brsmu.2018.085

ORIGINAL RESEARCH

The use of iron oxide magnetic nanospheres and nanocubes for targeted doxorubicin delivery into 4t1 mouse breast carcinoma cells

Nizamov TR1, Garanina AS1,2, Uvarova VI1,2, Naumenko VA1, Shchetinin IV3, Savchenko AG1
About authors

1 Laboratory of Biomedical Nanomaterials National University of Science and Technology MISiS, Moscow

2 Research Laboratory of Tissue-Specific Ligands, Faculty of Chemistry, Lomonosov Moscow State University, Moscow

3 Department of physical materials science, National University of Science and Technology MISiS, Moscow

Correspondеnce should be adressed: Timur R. Nizamov
Leninsky 4, Moscow, 119049; moc.liamg@rumit.vomazin

About paper

Funding: the study was supported by the Ministry of Education and Science of the Russian Federation in the context of the Agreement #14.578.21.0201 (project code RFMEFI57816X0201).

Received: 2018-08-28 Accepted: 2018-09-20 Published online: 2018-12-31
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Fig. 1. Microimages of iron oxide NS (1) and NC (2), histograms of size distribution and average hydrodynamic size of the particles
Fig. 2. Data yielded by the XRD analysis for NS (1) and NC (2)
Fig. 3. Magnetization curves for NS (1) and NC (2)
Fig. 4. The hydrodynamic size of NP after transfer to an aqueous phase: NS before (1) and after (3) doxorubicin loading; NC before (2) and after (4) doxorubicin loading.
Fig. 5. Toxicity of NP against the 4T1 cell line. The histogram shows cell survival after 48 h incubation with NC and NS (A); after 48-h incubation with NC-Dox, NS-Dox and free doxorubicin (B). The MTS assay. Results are presented as mean values ±SD. * p < 0.05; ** p < 0.01. Percentage of living cells incubated with the sodium phosphate buffer was taken as 100%
Fig. 6. Accumulation dynamics of free and entrapped doxorubicin in 4T1 cells. Fluorescence microscopy (A–J); a histogram showing the relationship between the intensity of doxorubicin fluorescence in the cells and the time of cell incubation with Dox, NC-Dox and NS-Dox (K). Results are presented as mean values ±SD. * p < 0.05; ** p < 0.01
Table. Results of the XRD analysis of NS and NC