2018 / 06

Next Paper
DOI: 10.24075/brsmu.2018.079

ORIGINAL RESEARCH

A novel spheroid model for preclinical intercellular nanophotosensitizer-mediated tumor study

Maklygina YuS1, Romanishkin ID1, Ryabova AV1, Yakavec IV2,3, Bolotin L2, Loschenov VB1,4
About authors

1 Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow

2 Research Centre for Automatic Control of Nancy, University of Lotaringia, Nancy, France

3 The Belarusian State University, Minsk, Belarus

4 National Research Nuclear University MEPhI, Moscow

Correspondence should be addressed: Yuliya S. Maklygina
Vavilova 38, Moscow, 119991; ur.usm.scisyhp@avonosmas.su

About paper

Funding: this work was supported by the Ministry of Education and Science of the Russian Federation (Agreement RFMEFI61618X0096 # 14.616.21.0096 of February 12, 2018).

Received: 2018-06-26 Accepted: 2018-10-23 Published online: 2018-12-30
|
Fig. 1. The stepwise scheme of experiment with spheroid model and AlPc NPs
Fig. 2. Image of AlPc NP fluorescence flaring up over the time. Autofluorescence (green) excitation at λex ~ 488 nm, AlPc NPs fluorescence (red) excitation at λex ~ 633 nm: 15 min (A); 20 min (B); 30 min (C); 40 min (D); 50 min (E); 1 h (F)
Fig. 3. The imaging of digital separation signals of (A) spectral image converted to RGB colors and (B) AlPc NPs fluorescence distribution. C. The fluorescence spectra recorded with the curve color corresponding to the highlighted areas. The spectra are normalized on each area’s surface
Fig. 4. The graphiс representation of AlPc NPs uptake in the 4 spheroid sections: (A) 0°; (B) 90°; (C) 45°; (D) –45°. It was calculated only from the PS fluorescence λfl ~ 670 nm (λex ~ 633 nm), excluding cell’s autofluorescnence
Fig. 5. Comparison of AlPc fluorescence before PDT (A) vs after PDT (B). Autofluorescence excitation λ ~ 488 nm, AlPc NPs fluorescence excitation λ ~ 633 nm.
Fig. 6. Cell viability analysis after PDT, stained with acridine orange (AO) (green — living cells) (A) and propidium iodide (PI) (red — dead cells) (B). Excitation of AO fluorescence with 488 nm laser, excitation of 561 nm laser